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BCMBJHU SOM

Faculty & Research

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Takanari Inoue

Department Affiliation Primary: Cell Biology
Secondary: Biological Chemistry
RankAssociate Professor
Phone Numbers443-287-7668
Fax: 410-614-8375
Emailjctinoue@jhmi.edu
School of Medicine Address855 N. Wolfe Street
453 Rangos
Baltimore MD 21205
Link to Lab Homepage
Takanari Inoue

Research Topic: Synthetic cell biology: total synthesis of cellular functions such as neutrophil chemotaxis and ciliary mechano-sensation

Research Focus 

Our research focuses on “synthetic cell biology” to dissect and reconstitute intricate signaling networks. In particular, we investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally dynamic information processing at various compartments in living cells. In parallel, our lab also tries to understand how cell morphology affects biochemical functions. Ultimately, we will generate completely orthogonal nano-machinery in artificial cells that can achieve existing, and even novel, cellular functions. 

Message for BCMB Students

Our research is conducted in an open lab style building which embodies a multidisciplinary research. Students who are enthusiastic about learning diverse disciplines, developing innovative techniques and challenging fundamental biological problems would enjoy the environment. Students are lways welcomed to our lab for discussions about potential research projects.

 

 

Publications:

Miyamoto T., Rho E, Sample V., Akano H., Magari M., Ueno T., Chen M., Tokumitsu H., Zhang J., and Inoue T. “Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators” Cell Reports 11, 657-670 (2015)
PubMed Reference

Lin B., Yin T., Wu Y.I., Inoue T. and Levchenko A. “Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration” Nature Communications 6, 6619 (2015)
Note: Press release: “A digital field guide to cancer cells”
PubMed Reference

Adams P.J., Johny M.B., Dick I.E., Inoue T., and Yue D.T., “Apocalmodulin itself promotes ion channel opening and Ca2+ regulation” Cell 159 (3), 608-622 (2014)
PubMed Reference

Razavi S., Su S. and Inoue T. “Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices” ACS Synthetic Biology 3 (9), 676-685 (2014)
Note: “Introducing Authors” ACS Synthetic Biology
PubMed Reference

Onuma H., Komatsu T., Arita M., Hanaoka K., Ueno T., Terai T., Nagano T., and Inoue T. "Rapidly rendering cells phagocytic through a cell-surface display technique and concurrent Rac activation" Science Signaling, 7, rs4, 1-7 (2014)
Note: “Cover Story” Science Signaling, “Turning cells into garbage collector” Editor’s Choice in Science, Interviewed and broadcasted by FOX45 NEWS (https://drive.google.com/file/d/0B-wUSlcBaaEsSmpmQmhIblMzTXc/view?usp=sharing), Interview for “Thought Leaders” series by Medical-News, Press release: MOLECULAR “EAT NOW” SIGNAL MAKES CELLS DEVOUR DYING NEIGHBORS, “Training Cells to Devour Dying Neighbors” highlighted in NIGMS Biomedical Beat Blog, Highlighted in 5th Annual Henrietta Lacks Memorial Lecture, “Designer Immune Cells” highlighted in 2014 Research Highlights from Johns Hopkins Medicine (https://www.youtube.com/watch?v=Lb58GUNP5Qk)
PubMed Reference 

Suarez A, Ueno T, Huebner R, McCaffery JM, and Inoue T “Bin/Amphiphysin/Rvs (BAR) family members bend membranes in cells” Scientific Reports 4, (4693) 1-6 (2014)
PubMed Reference

Kobayashi T, Kim S, Lin YC, Inoue T, and Dynlacht BD. “CP110-interacting proteins, Talpid3 and Cep290, play overlapping and distinct roles in cilia assembly” Journal of Cell Biology 204, 215-229 (2014)
PubMed Reference
   
Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda . and Inoue T. “Genetically encoded calcium indicator illuminates calcium dynamics in cilia” Nature Methods 10, 1105-1107 (2013)Note: “Author File” Nature Methods
PubMed Reference

Thevathasa, JV, Tan E, Hui Z, Lin YC, Li Y. Inoue T and Fivaz M. “Local positive feedback from PI3K to Ras drives cell polarization and migration” Molecular Biology of the Cell (doi:10.1091/mbc.E12-12-0905)
PubMed Reference

Lin YC, Su S. and Inoue T. “Visualizing Molecular Diffusion through Passive Permeability Barriers in Cells: Conventional and Novel Approaches“ Current Opinion in Chemical Biology 2013 (doi:10.1016/j.cbpa.2013.04.027)
PubMed Reference

Lin YC, Liu TY, Razavi S and Inoue T. “Rapidly Reversible Manipulation of Molecular Activities Using Dual Chemical Dimerizers” Angewandte Chemie 52, 6450 (2013)
PubMed Reference

Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi T, and Inoue T. “Chemically-inducible diffusion trap reveals molecular sieve-like barrier at primary cilia“ Nature Chemical Biology 9, 437 (2013)
PubMed Reference

Note: “Research on cilia heats up: Implications for hearing vision loss and kidney disease” Press release, Featured in “ChemBioVault”, “New cilia research could have implications for kidney disease” (American Society of Nephrology, In The Loop), “New clues about molecular composition of cilia” MIGMS Latest News, “Cilia Admit Large Cytosolic Proteins”, Kidney News, “Signaling: Shifting at Ciliary Base” News & Views, Nature Chemical Biology

DeRose R,  Miyamoto T and Inoue T. “Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology“ Pfülgers Archiv 465, 409-417 (2013)
PubMed Reference

Miyamoto T, Razavi S, DeRose R, and Inoue T. “Synthesizing Biomolecule-based Boolean Logic Gates” ACS Synthetic Biology 2, (2), 72-82 (2013)
Note: “Podcast” (ACS Synthetic Biology)
PubMed Reference

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