Deborah Andrew

Image of Dr. Debbie Andrew

Deborah Andrew

Primary Appointment: 
Cell Biology
Office: 410-614-2722
Lab: 410-614-2645

725 N. Wolfe Street
Hunterian G10
Baltimore, MD  21205

Research topic: 

Organogenesis in a model system: Formation of the Drosophila salivary gland and trachea

Tube formation is a ubiquitous process required to sustain life in all multicellular organisms. Tubular organs in humans include the lungs, vasculature, digestive and excretory systems, as well as secretory organs such as the pancreas, salivary, prostrate, and mammary glands. My lab studies the Drosophila trachea and salivary gland as model systems for tube formation to learn how organ size, shape and function are normally controlled. Over the past several years, we have identified many genes expressed in the trachea and salivary gland, and are characterizing the subset required for early tube morphogenesis using a wide variety of genetic, imaging and biochemical approaches. Among the genes we have characterized are two that encode transcription factors required for internalization of either the trachea or salivary gland, trachealess and fork head. Three other genes we have characterized encode transcription factors controlling tube elongation, ribbon, huckebein and hairy. Finally, several genes encode components of signaling pathways required for organ positioning. Current efforts in the lab are directed toward identifying and characterizing the morphogenetic roles of the downstream effector molecules regulated by these transcription factors and signaling pathways. We also study the mechanisms whereby the salivary gland becomes specialized for secretion and have learned that secretory capacity in this and other secretory organs is largely controlled by a single transcription factor, CrebA. Finally, we are beginning to leverage what we have learned about the Drosophila salivary gland to compromise mosquito salivary glands as one approach to limiting malarial transmission.

BCMB students currently in the lab:
Selected Publications: 

Wells, M.B., Villamor, J. and Andrew, D.J. (2017) Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae. Scientific Reports. 7:601. PMID: 28377572

Chung, S., Kim, S., and Andrew, D.J. (2017) Uncoupling apical constriction from tissue invagination. Elife e22235. PMID: 28263180 PMCID: PMC5338918

Hanlon, C.D. and Andrew, D.J. (2016) Drosophila FoxL1 non-autonomously coordinates organ placement during embryonic development. Dev Biol 419: 273-284. PMID: 27618755 PMCID: PMC5237845

Loganathan, R., Cheng, Y.L., and Andrew, D.J. (2016) Development of the Drosophila Respiratory System. Book Chapter in “Organogenetic gene networks: Genetic control of Organ Function”. Ed. J. Castelli-Gair and P. Bovolenta. Springer. p151-212

Loganathan, R., Lee, J.S., Wells, M.B., Grevengoed, E., Slattery, M. and Andrew, D.J. (2016) Ribbon regulates morphogenesis of the Drosophila embryonic salivary gland through transcriptional activation and repression. Dev Biol 409: 234-250. PMID: 26477561 PMCID: PMC4750396.

Cheng, Y.L. and Andrew, D.J. (2015). Extracellular Mipp1 activity confers migratory advantage to epithelial cells during collective migration. Cell Reports 13:2174-88. PMID: 26628373 PMCID: PMC4776642

Wells, M.B. and Andrew, D.J. (2015) Salivary gland cellular architecture in the Asian malaria vector mosquito Anopheles stephensi. Parasites and Vectors 8:617. PMID: 26627194 PMCID: PMC4667400

Hanlon, C.D. and Andrew, D.J. (2015) Outside-in signaling - a brief review of GPCR signaling with a focus on the Drosophila GPCR family. J Cell Sci 128: 3533-3542. PMID: 26345366 PMCID: PMC4610211

Andrew, D.J. and Yelon, D. (2015) Developmental mechanisms, patterning and organogenesisis. Curr Opin Genet Dev. Epub 2015 Jun 4.

Chung, S.-Y. and Andrew, D.J. (2014) Cadherin 99C regulates apical expansion and cell rearrangement during epithelial tube elongation. Development 141:1950-60. PMID: 24718992 

Fox, R.M., Vaishnavi, A., Maruyama, R., and Andrew, D.J. (2013) Organ-specific gene expression: the bHLH protein Sage provides tissue-specificity to Drosophila FoxA. Development 140: 2160-71. PMID: 23578928

Ismat, A., Cheshire, A. and Andrew, D.J. (2013) The secreted AdamTS-A metalloprotease is required for collective cell migration. Development 140:  1981-1993. PMID: 23578928

Chung, S.-Y., Chavez, C. and Andrew, D.J. (2011) Trachealess (Trh) regulates all tracheal genes during Drosophila embryogenesis.   Developmental Biology 360: 160-172.  PMID: 21963537

Fox, R.M., Hanlon, C.D. and Andrew, D.J. (2010) The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity. Journal of Cell Biology 191: 479-492. PMID: 21041443

Chung, S.-Y., Vining, M.S., Bradley, P.L., Chan, C.-C., Wharton, K.A. and Andrew, D.J. (2009) Serrano (Sano) functions with the planar cell polarity genes to control tracheal tube length  PLoS Genetics 5(11): e1000746. PMID: 19956736

Kerman, B.E., Cheshire, A.M., Myat, M.M., and Andrew, D.J. (2008) Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin.  Developmental Biology 320:  278-288. PMID: 18585700

Abrams, E.W., Mihoulides, W.K. and Andrew, D.J. (2006) Fork head and Sage maintain a uniform and patent salivary gland lumen through regulation of two downstream target genes, PH4SG1 and PH4SG2. Development 133: 3517-3527. PMID: 16914497

Myat, M.M. and Andrew, D.J. (2002) Epithelial tube morphology is determined by the polarized growth and delivery of apical membrane.  Cell 111: 879-891. PMID: 12526813

Bradley, P.L. and Andrew, D.J. (2001) ribbon encodes a BTB-containing transcription factor required for directed cell migration.  Development 128: 3001-3015. PMID: 11532922

Myat, M.M. and Andrew, D.J. (2000) FORK HEAD prevents apoptosis and promotes cell shape change during formation of the Drosophila salivary glands.  Development 127: 4217-4226. PMID: 10976053

Myat, M.M. and Andrew, D.J. (2000) Organ shape in the Drosophila salivary gland is controlled by regulated, sequential internalization of the primordia.  Development 127: 679-691. PMID: 10648227

Isaac, D.D. and Andrew, D.J. (1996) Tubulogenesis in Drosophila:  A requirement for the trachealess gene product.  Genes and Development 10: 103-117. PMID: 8557189