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Bloomberg Distinguished Professor
Molecular Biology and Genetics
725 N. Wolfe Street
Baltimore MD 21205
Mechanisms of Ribosome Translation and Translational Control
The ribosome is the macromolecular complex that translates the genetic code into functional polypeptides. We are interested in how this ribonucleoprotein (RNA + protein) machine catalyzes and coordinates the complex molecular events of translation. Current work in the Green lab ranges all the way from translational initiation mechanisms in bacteria to ribosome homeostasis in human disease. In all of our projects, we rely on biochemical methods, using in vitro translation systems fully reconstituted from bacterial and yeast components. Most projects in the lab also incorporate ribosome profiling and other high throughput genome-wide approaches to leverage our biochemical findings into biological insights. In the bacterial system, we are interested in how the cell decides which mRNAs to translate, how pauses during elongation are resolved, and how irreversibly arrested ribosomes are rescued and recycled. In eukaryotes, we are interested in the lifetime of an mRNA: its synthesis and processing in the nucleus, its translation by the ribosome in the cytoplasm, its ultimate degradation, and how the ribosome participates in these decisions – these questions are essentially those of gene regulation and mRNA quality control. Finally, we are interested in how ribosome homeostasis is critical to gene expression, and thus ultimately to human health and disease. These questions revolve around the ribosome and the cellular factors that engage it; these are the molecular problems that fascinate us.
Schuller AP, Wu CC, Dever TE, Buskirk AR, Green R. (2017) eIF5A Functions Globally in Translation Elongation and Termination. Mol Cell. 2017 Apr 20;66(2):194-205.e5.
Mills EW, Green R, Ingolia NT. (2017) Slowed decay of mRNAs enhances platelet specific translation. Blood 2017. Apr 27; 129(17): e38-e48.
Guydosh NR, Green R. (2017) Translation of poly(A) tails leads to precise mRNA cleavage. RNA 2017. 23: 749-761.
Mills EW, Wangen J, Green R, Ingolia NT. (2016) Dynamic regulation of a ribosome rescue pathway in erythroid cells and platelets. Cell Reports. 2016 Sep 27;17(1):1-10.
Radhakrishnan A, Chen YH, Martin S, Alhusaini N, Green R, Coller J. (2016) The DEAD-Box Protein Dhh1p Couples mRNA Decay and Translation by Monitoring Codon Optimality. Cell. 167:1-11.
Mohammad F, Woolstenhulme CJ, Green R, Buskirk AR. (2016) Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling. Cell Rep. 2016 Feb 2;14(4):686-94.
Young DJ, Guydosh NR, Zhang F, Hinnebusch AG, Green R. (2015) Rli1/ABCE1 Recycles Terminating Ribosomes and Controls Translation Reinitiation in 3'UTRs In Vivo. Cell. 162(4):872-84.
Woolstenhulme CJ, Guydosh NR, Green R, Buskirk AR. (2015) High-precision analysis of translational pausing by ribosome profiling in bacteria lacking EFP. Cell Rep. 11(1):13-21.
Koutmou KS, Schuller AP, Brunelle JL, Radhakrishnan A, Djuranovic S, Green R. (2015) Ribosomes slide on lysine-encoding homopolymeric A stretches. Elife. 2015 Feb 19;4. doi: 10.7554/eLife.05534.
Guydosh, NR and Green, R. (2014) Dom34 rescues ribosomes in 3' untranslated regions. Cell 156:950-62.