Robert N. Cole

Image of Dr. Robert Cole

Robert N. Cole

Assistant Professor
Additional Title: 
Director of Mass Spectrometry and Proteomics Facility
Primary Appointment: 
Biological Chemistry

733 N. Broadway Street
371 MRB
Baltimore MD 21205

Research topic: 

Mass spectrometry strategies to characterize proteins and their modifications in malnutrition, neurological diseases and cancer

Selected Publications: 

Watkins EE, Arbez N, Waldron-Roby E, O'Meally R, Ratovitski T, Cole RN, and Ross CA (2014) Phosphorylation of mutant huntingtin at serine 116 modulates neuronal toxicity. PLoS One 9: e88284.

Herbrich SM, Cole RN, West KP, Jr., Schulze K, Yager JD, Groopman JD, Christian P, Wu L, O'Meally RN, May DH, McIntosh MW, and Ruczinski I (2013) Statistical Inference from Multiple iTRAQ Experiments without Using Common Reference Standards.  Journal of Proteome Research 12: 594-6

Foster DB, Liu T, Rucker J, O'Meally RN, Devine LR, Cole RN, and O'Rourke B (2013) The cardiac acetyl-lysine proteome.  PLoS One 8: e67513.

Cole RN, Ruczinski I, Schulze K, Christian P, Herbrich S, Wu L, Devine LR, O'Meally RN, Shrestha S, Boronina TN, Yager JD, Groopman J, and West KP, Jr. (2013) The plasma proteome identifies expected and novel proteins correlated with micronutrient status in undernourished Nepalese children. Journal of Nutrition 143: 1540-1548.

Mattis VB, Svendsen SP, Ebert A, Svendsen CN, King AR, Casale M, Winokur ST, Batugedara G, Vawter M, Donovan PJ, Lock LF, Thompson LM, Atwal RS, Zhu Y, Fossale E, Gillis T, Mysore J, Li JH, Seong I, Shen Y, Chen X, Wheeler VC, MacDonald ME, Gusella JF, Akimov S, Arbez N, Juopperi T, Ratovitski T, Chiang JH, Kim WR, Chighladze E, Watkin E, Zhong C, Makri G, Cole RN, Margolis RL, Song H, Ming G, Ross CA, Kaye JA, Daub A, Sharma P, Mason AR, Finkbeiner S, Rushton D, Brazier SP, Battersby AA, Redfern A, Tseng HE, Harrison AW, Kemp PJ, Allen ND, Castiglioni V, Onorati M, Cattaneo E, Yu J, Thomson JA, and Arjomand J (2012) Induced pluripotent stem cells from patients with Huntington's disease show CAG-repeat-expansion-associated phenotypes.  Cell Stem Cell 11: 264-278.

Murray CI, Uhrigshardt H, O'Meally RN, Cole RN, and Van Eyk JE (2012) Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay.  Molecular and Cellular Proteomics 11: M111 013441.

Ratovitski T, Chighladze E, Arbez N, Boronina T, Herbrich S, Cole RN, and Ross CA (2012) Huntingtin protein interactions altered by polyglutamine expansion as determined by quantitative proteomic analysis.  Cell Cycle 11: 2006-2021.

Zizak M, Chen T, Bartonicek D, Sarker R, Zachos NC, Cha B, Kovbasnjuk O, Korac J, Mohan S, Cole RN, Chen Y, Tse CM, and Donowitz M (2012) Calmodulin kinase II constitutively binds, phosphorylates, and inhibits brush border Na+/H+ exchanger 3 (NHE3) by a NHERF2 protein-dependent process. Journal of Biological Chemistry 287: 13442-13456.

Cammarato A, Ahrens CH, Alayari NN, Qeli E, Rucker J, Reedy MC, Zmasek CM, Gucek M, Cole RN, Van Eyk JE, Bodmer R, O'Rourke B, Bernstein SI, and Foster DB (2011) A Mighty Small Heart: The Cardiac Proteome of Adult Drosophila melanogaster.  PLoS One 6: e18497.

Hartman IZ, Kim A, Cotter RJ, Walter K, Dalai SK, Boronina T, Griffith W, Lanar DE, Schwenk R, Krzych U, Cole RN, and Sadegh-Nasseri S (2010) A reductionist cell-free major histocompatibility complex class II antigen processing system identifies immunodominant epitopes.  Nature Medicine 16: 1333-1340.

Ratovitski T, Gucek M, Jiang H, Chighladze E, Waldron E, D'Ambola J, Hou Z, Liang Y, Poirer MA, Hirschhorn RR, Graham R, Hayden MR, Cole RN, and Ross CA (2009) Mutant Huntingtin N-terminal fragments of specific size mediate aggregation and toxicity in neuronal cells.  Journal of Biological Chemistry 284: 10855-10867.

Guo Y, Singleton PA, Rowshan A, Gucek M, Cole RN, Graham DR, Van Eyk JE, and Garcia JG (2007) Quantitative proteomic analysis of human endothelial cell membrane rafts: Evidence of MARCKS and MRP regulation in the sphingosine 1-phosphate-induced barrier enhancement.  Molecular and Cellular Proteomics 6: 689-696.

Wells L, Vosseller K, Cole RN, Cronshaw JM, Matunis MJ, and Hart GW (2002) Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications.  Molecular and Cellular Proteomics 1: 791-804.

Dinkova-Kostova AT, Holtzclaw WD, Cole RN, Itoh K, Wakabayashi N, Katoh Y, Yamamoto M, and Talalay P (2002) Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants.  Proceedings of the National Academy of Sciences, USA 99: 11908-11013.

Cole RN and Hart GW (2001) Cytosolic O-glycosylation is abundant in nerve terminals.  Journal of Neurochemistry 79: 1080-1089.