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Pharmacology and Molecular Sciences; IBBS Epigenetics Center
855 N. Wolfe Street
Baltimore MD 21205
Epigenetic pathways in disease, histone and chromatin modifications, and small RNA directed gene silencing
Eukaryotic cells package their genomes in the form of chromatin, which is comprised of histone proteins and DNA. Modification of chromatin by chemical marks such as methylation and acetylation affects how cellular machineries interpret the genome. The Taverna laboratory studies how histone marks contribute to an “epigenetic/histone code” that may dictate chromatin-templated functions like transcriptional activation and gene silencing, as well as how these On/Off states are inherited/ propagated. For example, transcription-modulating protein complexes with PHD finger motifs (methyl lysine “readers”) or Bromodomains (acetyl lysine “readers”) often have enzymatic activities that “write” these same histone marks. To explore these connections we use biochemistry and cell biology in a variety of model organisms ranging from mammals to yeast and ciliates. The lab also investigates links between small RNAs and histone marks involved in gene silencing. Importantly, many histone binding proteins have clear links to human disease, notably leukemia and other cancers.
Papazyan R, Taverna SD. Separation and purification of multiply acetylated proteins using cation-exchange chromatography. Methods Mol Biol. 981:103-13, 2013.
Byrum SD, Raman A, Taverna SD, Tackett AJ. ChAP-MS; a method for identification of proteins and histone posttranslational modifications at a single genomic locus. Cell Rep. 2(1):198-205, 2012.
Taverna SD, Cole PA. Drug discovery: Reader's block. Nature. 468(7327):1050-1, 2010.
Taverna SD, Ueberheide BM, Liu Y, Tackett AJ, Diaz R, Shabanowitz J, Chait BT, Hunt DF and Allis CD. Long-distance combinatorial linkage between methylation and acetylation on H3 N-termini. Proc. Natl. Acad. Sci. USA. 104:2086-2091, 2007.
Taverna SD, Li H, Ruthenburg AJ, Allis CD and Patel DJ. How chromatin binding modules interpret histone modifications. Nat. Struct. Mol. Bio. 14:1025-1040, 2007.
Taverna SD, Ilin S, Rogers RS, Tanny JC, Lavender H, Li H, Baker L, Boyle J, Blair LP, Chait BT, Patel DJ, Aitchison JD, Tackett AJ, and Allis .D. Yng1 PHD finger binding to histone H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. Molecular Cell. 24:785-796, 2006.
Taverna S.D., Coyne RS, and Allis CD. Methylation of histone H3 at lysine 9 targets programmed DNA elimination in Tetrahymena. Cell 110:701-711, 2002.
Jacobs SA, Taverna SD, Zhang Y, Briggs SD, Li J, Eissenberg JC, Allis CD, and Khorasanizadeh S. (2001) Specificity of the HP1 chromodomain for the methylated N-terminus of histone H3. EMBO J. 20:5232-5241, 2001.