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Molecular Biology and Genetics
725 N. Wolfe Street
Baltimore MD 21205
Developmental genetics: RNA and protein dynamics in embryos
Our lab studies the earliest stages of embryogenesis to understand how single-celled eggs develop into complex multicellular embryos. We study how RNAs and proteins become localized to pattern the developmental potential of newly fertilized eggs. We have identified a family of intrinsically-disordered proteins that form gel-like assemblies that sequester RNA (RNA granules) in the cytoplasm. We use genetics, microscopy and biochemistry to identify, observe and reconstitute RNA granules in and out of cells. We have also developed methods for scalable and seamless genome editing using CRISPR/Cas9.
Putnam A, Cassani M, Smith J, Seydoux G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. doi: 10.1038/s41594-019-0193-2.
Paix A, Folkmann A, Goldman DH, Kulaga H, Grzelak MJ, Rasoloson D, Paidemarry S, Green R, Reed RR, Seydoux G. Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10745-E10754. doi: 10.1073/pnas.1711979114.
Smith J, Calidas D, Schmidt H, Lu T, Rasoloson D, Seydoux G. Spatial Patterning of P granules by RNA-induced phase separation of the intrinsically disordered protein MEG-3. eLife. 2016 Dec 3;5. doi: 10.7554/eLife.21337
Wang, J. T., Smith, J. , Chen, B. C., Schmidt, H., Rasoloson, D., Paix, A. , Lambrus, B. G., Calidas, D., Betzig, E. and Seydoux, G. (2014) . Regulation of RNA granule dynamics by phosphorylation of serine-rich, intrinsically-disordered proteins in C. elegans. eLife, Dec 23;4. doi: 10.7554/eLife.04591.
Paix, A. , Wang, Y. Lee, CY S., Calidas, D., Lu, T. Smith, J., Schmidt, H., Seydoux, G. (2014) A scalable method for seamless genome editing by homology-directed repair of Cas9-induced cuts. Genetics, Vol. 198, 1–10 December 2014. doi: 10.1534/genetics.114.170423
Griffin E , Odde D and Seydoux G. Regulation of the MEX-5 gradient by a spatially-segregated kinase-phosphatase cycle (2011). Cell, 16;146(6):955-68. PubMed Reference
Gallo C, Wang J, Motegi F, Seydoux,G. (2010). Cytoplasmic partitioning of P granule components is not required to specify the germline in C. elegans. Science, 17;330(6011):1685-9.