733 N. Broadway
Miller Research Bldg., Room 323
Baltimore, MD 21287
Our lab focuses on (1) elucidating cellular mechanisms of synaptogenesis in mouse cortex, (2) developing novel technologies that selectively label and control behaviorally-relevant circuits, and (3) dissecting mechanisms of flexible cognitive action controls into cellular level. The first project uses fine-scale, optical techniques that create spatiotemporally-controlled patterns of neurotransmitter release in the dendrite, such that local triggering and signaling mechanisms of synapse formation can be studied. New tools developed in the second project will allow us to map out the activity profile of individual neurons in behaving animals and test their causal relationship with specific behaviors. Such a tool would dramatically increase our knowledge of the pathways that underlie complex vertebrate behavior. Third project will define organizing principles of cognitive learning at cellular resolution.
Project 1: Cellular principles underlying synapse formation in developing and mature brain.
Project 2: Developing tools that link neural circuits to behaviors.
Project 3: Dissection of flexible cognitive behaviors at cellular resolution.
Kim J, Lee S, Jung K, Oh WC, Kim N, Son S, Jo Y, Kwon HB*, Heo WD*., (2019) Intensiometric biosensors visualize multiple small GTPases activities in vivo. Nature Communications Jan 14; 10(1):211 *Co-corresponding authors
Yetman MJ, Washburn E, Hyun JH, Osakada F, Hayano Y, Zeng H, Callaway EM, Kwon HB, Taniguchi H., (2019) Intersectional monosynaptic tracing for dissecting subtype-specific organization of GABAergic interneuron inputs. Nature Neuroscience 22(3):492~502
Beas-Alvarez BS, Wright BJ, Skirzewwski M, Leng Y, Hyun JH, Koita O, Ringelberg N, Kwon HB, Buonanno A, and Penzo MA., (2018) Inhibitory control of a thalamic stress circuit. Nature Neuroscience 21(7):963~973
Sutton L, Orlandi C, Song C, Oh WC, Muntean B, Xie K, Filippini A, Xie X, Satterfield R, Yaeger J, Renner K, Young SM, Xu B, Kwon HB, Martemyanov KA., (2018) Orphan receptor GPR158 controls stress-induced depression. eLIFE Feb 8;7. pii: e33273. doi: 10.7554/eLife.33273. [Epub ahead of print]
Lee D*, Hyun JH*, Jung K, Hannan P, Kwon HB., (2017) A calcium- and light-gated switch to induce gene expression in activated neurons. Nature Biotechnology Sep; 35(9): 858-863 *equal contribution * Highlighted in Nature Methods * Highlighted in Nature Chemical Biology * News and Views in Nature Biotechnology
Lee D*, Creed M*, Jung K*, Stefanelli T, Wendler DJ, Oh WC, Mignocchi NL, Luscher C, Kwon HB., (2017) Temporally precise labeling and control of neuromodulatory circuits in mammalian brain. Nature Methods 14(5): 495-503 *equal contribution
Sigler A*, Oh WC*, Imig C*, Altas B, Kawabe H, Cooper BH, Kwon HB, Rhee JS, Brose N., (2017) Formation and Maintenance of Functional Spines in the Absence of Presynaptic Glutamate Release. Neuron 94(2):304-311 *equal contribution
Oh WC, Lutzu S, Castillo PE and Kwon HB., (2016) De novo synaptogenesis induced by GABA in the developing mouse cortex. Science. 353(6303): 1037-1040 * Highlighted in Nature Review Neuroscience
Kantevari S, Passlick S, Kwon HB, Richers M, Sabatini B, Ellis-Davies G., (2016) Development of anionically decorated caged neurotransmitters: in vitro comparison of 7-nitroindolinyl- and 2-(p-phenyl-o-nitrophenyl)-propyl-based photochemical probes. Chembiochem 17(10):953-61.
Kim T*, Oh WC*, Choi JH and Kwon HB., (2016) Emergence of functional subnetwork in layer 2/3 cortex induced by sequential spikes in vivo. Proc Natl Acad Sci U S A. Mar 8; 113(10): E1372-81 *equal contribution
Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E., (2016) Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. Neuron 89(4): 756-69.
Gross GG*, Junge JA*, Mora R*, Kwon HB, Olsen CA, Takahashi TT, Liman E, Sabatini BL, Roberts RW, Arnold DB., (2013) Recombinant probes for visualizing endogenous proteins in living cells. Neuron 78(6):971-985 *equal contribution
Olson JP, Kwon HB, Takasaki KT, Chiu CQ, Higley MJ, Sabatini BL, Ellis-Davies GCR., (2013) Optically Selective Two-photon Uncaging of Glutamate at 900nm. J. Am. Chem. Soc. 135(16): 5954~5957
Kwon HB, Kozorovitskiy Y, Oh WJ, Akhtar N, Saulnier JL, Gu C, Sabatini BL., (2012) Cortical synaptogenesis and excitatory synapse number are determined via a Neuroligin-1-dependent intercellular competition. Nature Neuroscience 15(12):1667~1674.
Peixoto R, McCoy PA, Kwon HB, Mabb AM, Sabatini BL, Philpot BD, Ehlers MD., (2012) Trans-Synaptic Signaling by Activity-Dependent Cleavage of Neuroligin-1. Neuron 76:396~409
Kwon HB and Sabatini BL., (2011) Glutamate induces de novo growth of functional spines in developing cortex. Nature 474 (7349):100-4. (PMCID: PMC3107907)
Mukherjee K, Yang X, Gerber SH, Kwon HB, Ho A, Castillo PE, Liu X, Südhof TC., (2010) Piccolo and bassoon maintain synaptic vesicle clustering without directly participating in vesicle exocytosis. Proc Natl Acad Sci U S A. 107(14):6504-9 (PMCID: PMC2851964)
Kaeser PS, Kwon HB, Chiu CQ, Deng L, Castillo PE, Südhof TC., (2008) RIM1 and RIM1 are synthesized from distinct promoters of the RIM1 gene to mediate differential but overlapping synaptic functions. J. Neurosci, 28: 13435-13447 (PMCID: PMC2701653)
Kwon HB and Castillo PE., (2008) Role of Glutamate Autoreceptors at Hippocampal Mossy Fiber Synapses. Neuron 60: 1082-1094
Kaeser PS, Kwon HB, Blundell J, Chevaleyre V, Morishita W, Malenka RC, Powell CM, Castillo PE, Südhof TC., (2008) RIM1 phosphorylation at serine-413 by protein kinase A is not required for presynaptic long-term plasticity or learning. Proc Natl Acad Sci U S A. 105(38):14680-5 (PMCID: PMC2567150)
Kwon HB and Castillo PE., (2008) Long-Term Potentiation Selectively Expressed by NMDA Receptors at Hippocampal Mossy Fiber Synapses. Neuron 57: 108-20 (PMCID: PMC2390917) * previewed by Angharad M. Kerr and Peter Jonas (2008) Neuron 57: 5-7
Chandra S, Fornai F, Kwon HB, Yazdani U, Atasoy D, Liu X, Hammer RE, Battaglia G, German DC, Castillo PE, Südhof TC., (2004) Double-knockout mice for alpha- and beta-synucleins: effect on synaptic functions. Proc Natl Acad Sci U S A 101: 14966-14971 (PMCID: PMC522043)
Kwon HB, Kim SH, Kim SE, Jang IH, Ahn Y, Lee WJ, Choi KY., (2002) Drosophila Extracellular Signal Regulated Kinase Involves in The Insulin Mediated Proliferation of Schneider Cells. J. Biol. Chem. 277: 14853-14858
Kim SH, Kwon HB, Kim YS, Ryu JH, Kim KS, Ahn Y, Lee WJ, Choi KY., (2002) Isolation and Characterization of A Drosophila Homologue of MKP-3 Which Has A High Substrate Specificity Toward ERK. Biochem. J. 361: 143-151 (PMCID: PMC1222289)