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Biophysics and Biophysical Chemistry
725 N. Wolfe Street
Baltimore MD 21205
Single-molecule and single-cell studies of cell division, chromosome conformation, transcription and gene regulation
My laboratory focuses on developing novel single-molecule imaging tools in live cells to probe various dynamic aspects of cellular processes. We develop single-molecule gene expression reporting systems and chromosomal DNA conformation markers to probe the dynamics of gene regulation and transcription in live bacterial cells. We also pioneered the use of superresolution imaging to probe the structure, function and dynamics of the bacterial cell division machinery. Our work is at the frontier of imaging dynamic cellular processes, and has enabled new quantitative understandings of gene regulation and cell division. Recently we are expanding our horizons by collaborating with experts of different fields to map the spatial organization of a single cell’s genome and epigenetic markers, and to develop new single-molecule based technologies for sensitive early detection of cancer markers in
Weng X, Bohrer, C.H., Bettridge K., Lagda, A.C., Cagliero, C., Jin, D. J., Xiao, J., Spatial organization of RNA polymerase and its relationship with transcription in E. coli, P.N.A.S., 2019, 10.1073/pnas.1903968116
Yang, X., Lyu, Z., Miguel, A., McQuillen, R., Huang, K.C., and Xiao, J., GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science, 2017. 355(6326): p. 744-747.
Coltharp, C., Buss, J., Plumer, T.M., and Xiao, J., Defining the rate-limiting processes of bacterial cytokinesis. PNAS, 2016. 113(8): p. E1044-53.
Buss J, Coltharp C., Shtengel G, Yang X, Hess H and Xiao J., A multi-layered protein network maintains the stability of the FtsZ-ring and modulate constriction dynamics in E.coli, PLoS Genetics, 2015, Apr 7;11(4):e1005128.
Hensel Z.,Weng X., Lagda A., Xiao J., Transcription factor mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells, 2013, PLoS Biology, 11(6): e1001591.
Hensel Z., Feng H., Han B., Hatem C., Wang J., Xiao J., Stochastic expression dynamics of transcription factor revealed by single-molecule noise analysis, Nat. Struct. Mol. Biol. 2012.