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Biophysics and Biophysical Chemistry
725 N. Wolfe Street
Baltimore MD 21205
Single-molecule and single-cell studies of cell division, chromosome conformation, transcription and gene regulation
Our research focuses on developing novel single-molecule imaging tools in live cells to probe the organization and dynamics of cellular processes, including gene regulation, chromosomal DNA conformation and cell division apparatus. Recently we expanded our horizons to map the spatial organization of a single cell’s genome and epigenetic markers, and to develop new single-molecule based technologies for sensitive early detection of cancer markers.
Yang, X., Lyu, Z., Miguel, A., McQuillen, R., Huang, K.C., and Xiao, J., GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ organizes septal cell wall synthesis. Science, 2017. 355(6326): p. 744-747.
Coltharp, C., Buss, J., Plumer, T.M., and Xiao, J., Defining the rate-limiting processes of bacterial cytokinesis. PNAS, 2016. 113(8): p. E1044-53.
Buss J, Coltharp C., Shtengel G, Yang X, Hess H and Xiao J., Al multi-layered protein network maintains the stability of the FtsZ-ring and modulate constriction dynamics in E.coli, PLoS Genetics, 2015, Apr 7;11(4):e1005128.
Hensel Z.,Weng X., Lagda A., Xiao J., Transcription factor mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells, 2013, PLoS Biology, 11(6): e1001591.
Hensel Z., Feng H., Han B., Hatem C., Wang J., Xiao J., Stochastic expression dynamics of transcription factor revealed by single-molecule noise analysis, Nat. Struct. Mol. Biol. 2012.
Guo F., Huang T., Buss J., Coltharp C., Hensel Z., Xiao J., In vivo structure of the E. coli FtsZ-ring revealed by photoactivated Localization Microscopy (PALM), PLoS One, 2010, 5, e12680.