855 N. Wolfe Street
Seeing is believing: My lab is interested in visualizing and quantifying biological events as they happen in situ in real-time. We use single-molecule imaging and spectroscopy technology in live cells, combined with theoretical modeling to extract dynamic biological information. Currently, the lab is working on how cells regulate gene expression. Gene expression has to be tightly controlled both temporally and spatially for an organism to survive and prosper. Dysfunction in the local regulation of gene expression is one of the most common sources of developmental and neurodegenerative disease. My laboratory focuses on this fundamental process by visualizing and manipulating the birth, trafficking, translation and decay of single RNA molecules in live cells and tissue. Recently we have developed a method called single-molecule imaging of nascent peptides (SINAPS) to visualize the translation of single mRNAs. With SINAPS, we can answer biological questions that are intractable before, such as the location of translation, the translation speed of ribosomes in cells, the initiation and release rate of ribosomes, the relationship between splicing, decay and translation, the effect of untranslated region on translation, etc. We are also interested in local translation in neuronal, such as the effect of synaptic stimulation and dendritic translation, and local translation in the axonal growth cone. Besides pure observation, my lab is also focused on developing optic and chemical methods to manipulate gene expression in live cells. Eventually, we hope to turn on or off the expression of a particular gene anywhere in a cell.
Wang S*, Latallo M.J.*, Zhang Z, Huang B, Bobrovnikov D, Dong D, Livingston N.M., Tjoeng W, Hayes L.R., Rothstein J.D., Ostrow L.W., Wu B‡, Sun S‡. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD. Nature Communications, 2021 Aug 13; 12(1):4908. doi: https://doi.org/10.1038/s41467-021-25082-9. PubMed PMID: 34389711.
Goldman D, Livingston N, Movsik J, Wu B†, Green R†, Live-Cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling, Mol Cell, 2021, 81, 1-11, DOI: 10.1016/j.molcel.2021.01.029. (†co-corresponding author)
Liu Y*, Zou R*, He S, Nihongaki Y, Li X, Razavi S, Wu B†, Ha T†, Very Fast CRISPR on Demand, Science, Vol 368, 2020, 1265-1269, DOI: 10.1126/science.aay8204 (*equal contribution, †co-corresponding author)
“Yoon Y.J.*, Bin Wu*, Buxbaum A.R., Sulagna D, Tsai A, English B.P., Grimm J.B., Lavis D.L. and Singer R.H., Glutamate-induced RNA localization and translation in neurons, Proceedings of National Academy of Science, 2016, (*equal contribution)”
Wu, B, Eliscovich C, Yoon Y and Singer R.H., Translation dynamics of single mRNAs in live cells and neurons, Science, Vol 352, Issue 6292, 2016
Wu, B. Buxbaum A.R., Katz Z, Yoon, Y and Singer R.H, Quantifying protein-mRNA interactions in single live cells, Cell, 2015, 162 (1): 211-220.
Wu, B*, Miskolci, V*, Sato H., Tutucci E., Kenworthy C.A., Donnelly S.K., Yoon Y.J., Cox, D, Singer R.H. and Hodgson L., Synonymous modification results in high fidelity gene expression of repetitive protein and nucleotide sequences, Genes & Developments, 2015, 29 (8): 876-886.