Image of Geraldine Seydoux

Geraldine Seydoux



725 N. Wolfe Street
706 PCTB
Baltimore MD 21205

Molecular Biology and Genetics

Our lab studies gene regulation in germ cells, with a focus on “RNA granules” which are phase-separated compartments rich in RNA and proteins. We have identified a family of intrinsically-disordered proteins that form gel-like assemblies that sequester RNA (RNA granules) in the cytoplasm. We use genetics, microscopy and biochemistry to identify, observe and reconstitute RNA granules in and out of cells.  We have also developed methods for scalable and seamless genome editing using CRISPR/Cas9.

Cassani M, Seydoux G. Specialized germline P-bodies are required to specify germ cell fate in Caenorhabditis elegans embryos. Development. 2022 Nov 1;149(21):dev200920. doi: 10.1242/dev.200920. Epub 2022 Nov 7. PMID: 36196602; PMCID: PMC9686995. PubMed

Ouyang JPT, Zhang WL, Seydoux G. The conserved helicase ZNFX-1 memorializes silenced RNAs in perinuclear condensates. Nat Cell Biol. 2022 Jul;24(7):1129-1140. doi: 10.1038/s41556-022-00940-w. Epub 2022 Jun 23. PMID: 35739318; PMCID: PMC9276528.  PubMed

Folkmann AW, Putnam A, Lee CF, Seydoux G. Regulation of biomolecular condensates by interfacial protein clusters. Science. 2021 Sep 10;373(6560):1218-1224. doi: 10.1126/science.abg7071. Epub 2021 Sep 9. PMID: 34516789; PMCID: PMC8627561.  PubMed

Putnam A, Cassani M, Smith J, Seydoux G. A gel phase promotes condensation of liquid P granules in Caenorhabditis elegans embryos. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. doi: 10.1038/s41594-019-0193-2. Epub 2019 Mar 4. Erratum in: Nat Struct Mol Biol. 2020 Feb;27(2):221. PMID: 30833787; PMCID: PMC6668929.  PubMed