Reza Kalhor
855 N. Wolfe St
Rangos 575
Baltimore, MD 21205
Our research seeks to map and engineer cell fate using synthetic biology and genomics approaches. To accomplish this objective, we develop: (A) molecular technologies that write cellular histories into their genomes, (B) sequencing strategies that read out written information with spatial resolution, and (C) computational methods that reconstruct the underlying biological processes based on recorded information. We apply these approaches to study embryogenesis, neurogenesis, and cancer metastasis, where we also aim to establish more effective cell therapies.
Fang W, Bell CM, Sapirstein A, Asami S, Leeper K, Zack DJ, Ji H†, Kalhor R†. Quantitative fate mapping: A general framework for analyzing progenitor state dynamics via retrospective lineage barcoding. Cell. 2022 Nov 23;185(24):4604-4620.e32. PMID: 36423582
Leeper K, Kalhor K, Vernet A, Graveline A, Church GM, Mali P, Kalhor R†. Lineage barcoding in mice with homing CRISPR. Nat Protoc. 2021 Apr;16(4):2088-2108. PMID: 33692551.
Lee HH*, Kalhor R*, Goela N*, Bolot J, Church GM. Terminator-free template-independent enzymatic DNA synthesis for digital information storage. Nat Commun. 2019 Jun 3;10(1):2383. PMID: 31160595.
Kalhor R†, Kalhor K, Mejia L, Leeper K, Graveline A, Mali P, Church GM†. Developmental barcoding of whole mouse via homing CRISPR. Science. 2018 Aug 31;361(6405). pii: eaat9804. PMID: 30093604.
Kalhor R, Mali P, Church GM. Rapidly evolving homing CRISPR barcodes. Nat Methods. 2017 Feb;14(2):195-200. PMID: 27918539.
Kalhor R, Tjong H, Jayathilaka N, Alber F, Chen L. Genome architectures revealed by tethered chromosome conformation capture and population-based modeling. Nat Biotechnol. 2012 Jan;30(1):90-8. PMID: 22198700.