855 N. Wolfe St
Baltimore, MD 21205
Our research seeks to better understand continuously dynamic biological processes, such as embryonic development and brain activity, by recording cells’ experiences in their genomes. To accomplish this objective, we develop: (A) molecular technologies that write cellular experiences into their nucleic acids, (B) sequencing strategies that read out written information with high spatial resolution, and (C) computational methods that reconstruct the underlying biological processes based on recorded information. We combine these recording approaches with genomics and epigenomics strategies to study early development, neurodevelopment, and brain function. Consequently, our work resides at the interface of biology and technology and involves both experimental and computational expertise.
Leeper K, Kalhor K, Vernet A, Graveline A, Church GM, Mali P, Kalhor R†. Lineage barcoding in mice with homing CRISPR. Nat Protoc. 2021 Apr;16(4):2088-2108. PMID: 33692551.
Lee HH*, Kalhor R*, Goela N*, Bolot J, Church GM. Terminator-free template-independent enzymatic DNA synthesis for digital information storage. Nat Commun. 2019 Jun 3;10(1):2383. PMID: 31160595.
Kalhor R†, Kalhor K, Mejia L, Leeper K, Graveline A, Mali P, Church GM†. Developmental barcoding of whole mouse via homing CRISPR. Science. 2018 Aug 31;361(6405). pii: eaat9804. PMID: 30093604.
Kalhor R, Mali P, Church GM. Rapidly evolving homing CRISPR barcodes. Nat Methods. 2017 Feb;14(2):195-200. PMID: 27918539.
Lee JH, Daugharthy ER, Scheiman J, Kalhor R, Yang JL, Ferrante TC, Terry R, Jeanty SS, Li C, Amamoto R, Peters DT, Turczyk BM, Marblestone AH, Inverso SA, Bernard A, Mali P, Rios X, Aach J, Church GM. Highly multiplexed subcellular RNA sequencing in situ. Science. 2014 Mar 21;343(6177):1360-3. PMID: 24578530.
Kalhor R, Tjong H, Jayathilaka N, Alber F, Chen L. Genome architectures revealed by tethered chromosome conformation capture and population-based modeling. Nat Biotechnol. 2012 Jan;30(1):90-8. PMID: 22198700.