725 N. Wolfe Street
Baltimore MD 21205
The ribosome is a complex molecular machine that translates the genetic code into functional polypeptides. Our work focuses on understanding how the ribosome functions at a molecular level and how changes in its activity lead to mRNA quality control and the induction of cellular stress responses. Work in the Green lab ranges widely in scope, from detailed mechanistic questions in ribosome rescue to surveying global changes in gene expression and dissecting the complex interplay of mammalian signaling pathways. We use a wide range of genetic, genomic, and biochemical approaches to explore these questions in bacteria, yeast, and increasingly in mammalian systems. Much of our ongoing work focuses on the importance of ribosome collisions in activating signaling pathways such as the integrated stress response (ISR) and various MAP kinase cascades.
Schuller AP, Wu CC, Dever TE, Buskirk AR, Green R. (2017) eIF5A Functions Globally in Translation Elongation and Termination. Mol Cell. 2017 Apr 20;66(2):194-205.e5.
Mills EW, Green R, Ingolia NT. (2017) Slowed decay of mRNAs enhances platelet specific translation. Blood 2017. Apr 27; 129(17): e38-e48.
Guydosh NR, Green R. (2017) Translation of poly(A) tails leads to precise mRNA cleavage. RNA 2017. 23: 749-761.
Mills EW, Wangen J, Green R, Ingolia NT. (2016) Dynamic regulation of a ribosome rescue pathway in erythroid cells and platelets. Cell Reports. 2016 Sep 27;17(1):1-10.
Radhakrishnan A, Chen YH, Martin S, Alhusaini N, Green R, Coller J. (2016) The DEAD-Box Protein Dhh1p Couples mRNA Decay and Translation by Monitoring Codon Optimality. Cell. 167:1-11.
Mohammad F, Woolstenhulme CJ, Green R, Buskirk AR. (2016) Clarifying the Translational Pausing Landscape in Bacteria by Ribosome Profiling. Cell Rep. 2016 Feb 2;14(4):686-94.
Young DJ, Guydosh NR, Zhang F, Hinnebusch AG, Green R. (2015) Rli1/ABCE1 Recycles Terminating Ribosomes and Controls Translation Reinitiation in 3’UTRs In Vivo. Cell. 162(4):872-84.
Woolstenhulme CJ, Guydosh NR, Green R, Buskirk AR. (2015) High-precision analysis of translational pausing by ribosome profiling in bacteria lacking EFP. Cell Rep. 11(1):13-21.
Koutmou KS, Schuller AP, Brunelle JL, Radhakrishnan A, Djuranovic S, Green R. (2015) Ribosomes slide on lysine-encoding homopolymeric A stretches. Elife. 2015 Feb 19;4. doi: 10.7554/eLife.05534.
Guydosh, NR and Green, R. (2014) Dom34 rescues ribosomes in 3′ untranslated regions. Cell 156:950-62.