Image of Takanari Inoue

Takanari Inoue

Professor, BCMB Program Director


855 N. Wolfe Street
476 Rangos
Baltimore MD 21205

Cell Biology

Biological Chemistry; Pharmacology and Molecular Sciences; Biomedical Engineering

Research Focus Our research focuses on “synthetic cell biology” to dissect and reconstitute intricate signaling networks. In particular, we investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally dynamic information processing at various compartments in living cells. In parallel, our lab also tries to understand how cell morphology affects biochemical functions. Ultimately, we will generate completely orthogonal nano-machinery in artificial cells that can achieve existing, and even novel, cellular functions.  Message for BCMB Students Our research is conducted in an open lab style building which embodies a multidisciplinary research. Students who are enthusiastic about learning diverse disciplines, developing innovative techniques and challenging fundamental biological problems would enjoy the environment. Students are lways welcomed to our lab for discussions about potential research projects.

Matsubayashi HT, Mountain J, Yao T, Peterson AF, Deb Roy A, Inoue T “Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosis” bioRxiv, 2022.12.31.522383

Deb Roy A, Gross EG, Pillai GS, Seetharaman S, Etienne-Manneville S, Inoue T “Non-catalytic allostery in α-TAT1 by a phospho-switch drives dynamic microtubule acetylation” Journal of Cell Biology 2022:221:11:e1-20

Watanabe S, Nihongaki Y, Itoh K, Watanabe S, Inoue T “Defunctionalizing Intracellular Organelles with Genetically-Encoded Molecular Tools Based on Engineered Phospholipase A/Acyltransferases” Nature Communications 2022:13:4413:1-15

Nakamura H, Rho E, Deng D, Razavi S, Matsubayashi HT, Inoue T “ActuAtor, a molecular tool for generating force in living cells” bioRxiv 2020.03.30.016360

Nihongaki Y, Matsubayashi HT, Inoue T. “A molecular trap inside microtubules probes luminal access by soluble proteins” Nature Chemical Biology (In Press)

Wu HD, Kikuchi M, Dagliyan O, Aragaki AK, Nakamura H, Dokholyan NV, Umehara T, Inoue T, “Rational design and implementation of a chemically inducible hetero-trimerization system” Nature Methods 2020;17: 928–936. Note: Research Highlight in Nature Chemical Biology, “Three’s company”

Kim AK, Wu HD, Inoue T, “Rational Design of a Protein Kinase A Nuclear-cytosol Translocation Reporter” Scientific Reports 2020:10:9365

Jacqueline Niu, Ivy E. Dick, Wanjun Yang, David Yue, Gordon F. Tomaselli, Inoue T*, Manu Ben Johny*. “Allosteric regulators selectively prevent Ca2+-feedback of CaV and NaV channels” eLife 2018;7:e35222a

Jacqueline Niu, Wanjun Yang, David T. Yue, Inoue T*, Manu Ben Johny*. “Duplex signaling by CaM and Stac3 enhances CaV1.1 function and provides insights into congenital myopathy” J. Gen. Phys. 2018;150:1145-1161

Shi-Rong Hong, Cuei-Ling Wang, Yu-Chen Chang, Ya-Chu Chang, Chun-Yu Lin, Ning Hsu, Hsiao-Chi Cheng, Yueh-Chen Chiang, Wei-En Huang, Nathan C. Shaner, Rajat Rohatgi, Inoue T*, Yu-Chun Lin* “Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling” Nature Communications 2018:9:1732:1-13; Note: Press release “Scientists Develop Method to Tweak Tiny ‘Antenna’ on Cells”

Nakamura H, Lee AA, Afshar AS, Watanabe S, Rho E, Razavi S, Suarez A, Lin YC, Tanigawa M, Huang B, DeRose R, Bobb D, Hong W, Gabelli SB, Goutsias J, Inoue T. “Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions” Nature Materials 2018;17:79-89.  Note: Press release “Researchers Report First-Ever “Hydrogels” Made in Living Cells”

Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Schurmans S, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T. “Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.” Cell 2017;168:264-279. Note: “Some Cells Need a ‘Haircut’ Before Duplicating” Press release, “Study Shows How and Why Hairlike Structures on Cells are Lost” PKD News Blog

Garcia-Gonzalo FR, Phua SC, Roberson EC, Galo Garcia III, Abedin M, Schurmans S, Inoue T*, Reiter JF. * “Ciliary Phosphoinositides Modulate Hedgehog Signaling.” Developmental Cell.  2015;34:400-409. Note: “Phosphoinositide Code for Primary Cilia” Preview by Developmental Cell, “Ciliary phosphoinositides regulate Hedgehog signaling” Highlight by Nature Reviews Molecular Cell Biology

Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Chen M,, Tokumitsu H, Zhang J, Inoue T. “Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators.” Cell Reports.  2015;11:657-670. Note: Press release: “Molecular spies sabotage a protein’s activities in specific cellular compartments”

Lin B, Yin T, Wu YI, Inoue T*, Levchenko A. * “Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.” Nature Commun.  2015;6:6619. Note: Press release: “A digital field guide to cancer cells”

Razavi S, Su S, Inoue T. “Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices.” ACS Synthetic Biol.  2014;3(9):676-685.