Takanari Inoue



855 N. Wolfe Street
476 Rangos
Baltimore MD 21205

Cell Biology

Biological Chemistry; Pharmacology and Molecular Sciences

Research Focus  Our research focuses on “synthetic cell biology” to dissect and reconstitute intricate signaling networks. In particular, we investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally dynamic information processing at various compartments in living cells. In parallel, our lab also tries to understand how cell morphology affects biochemical functions. Ultimately, we will generate completely orthogonal nano-machinery in artificial cells that can achieve existing, and even novel, cellular functions.  Message for BCMB Students Our research is conducted in an open lab style building which embodies a multidisciplinary research. Students who are enthusiastic about learning diverse disciplines, developing innovative techniques and challenging fundamental biological problems would enjoy the environment. Students are lways welcomed to our lab for discussions about potential research projects.    

Kim AK, Inoue T. “Rational design of a synthetic farnesyl-electrostatic switch based on hypervariable region of K-Ras4b” bioRxiv link: https://doi.org/10.1101/635839

Kim AK, Inoue T. “Rational Design of a Protein Kinase A Nuclear-cytosol Translocation Reporter” bioRxiv link: https://doi.org/10.1101/709766

Georgess D, Padmanaban V, Sirka O, Coutinho K, Choi A,  Frid G, Neumann N, Inoue T, and Ewald A. “Twist1-induced epithelial dissemination requires Prkd1 signaling” Cancer Research (Accepted on August 30th, 2019)

Kremer L, Hennes E, Brause A, Ursu A, Robke L, Matsubayashi HY, Nihongaki Y, Mejdrová I, Eickhoff J, Baumann M, Nencka R, Janning P, Kordes S, Schöler HE, Sterneckert J, Inoue T, Ziegler S, Waldmann H. “Discovery of the Hedgehog Pathway Inhibitor Pipinib that Targets PI4KIIIß” Angewandte Chemie (Accepted on August 15th, 2019)

Nakamura H, DeRose R, Inoue T.* “Harnessing biomolecular condensates in living cells” Journal of Biochemistry 2019; 166 (1): 13-27 Note: Selected for Cover Article Miao YC, Bhattacharya S, Banerjee T, Abubaker-Sharif, B, Long, Y, Inoue T, Devreotes PN, Iglesias PA “Wave patterns organize cellular protrusions and control cortical dynamics” Molecular Systems Biology 2019:15:e8585

Niu J, Yang W, Yue DT, Inoue T*, Johny* MB. “Duplex signaling by CaM and Stac3 enhances CaV1.1 function and provides insights into congenital myopathy” J. Gen. Phys. 2018;150:1145-1161

Niu J, Dick  IE, Yang W, Yue D, Tomaselli GF, Inoue T*, Johny* MB. “Allosteric regulators selectively prevent Ca2+-feedback of CaV and NaV channels” eLife 2018;7:e35222a

Wang C-L, Chang Y-C, Chang Y-C, Hong S-R, Lin CY,  Hsu N, Cheng H-C, Chiang Y-C, Huang W-E, Shaner NC, Rohatgi R, Inoue T*,  Lin YC* “Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling” Nature Communications 2018:9:1732:1-13

Nakamura H., Lee A.A., Afshar A.S., Lin Y-C., Tanigawa M., Suarez A., Razavi S., DeRose R., Bobb D., Hong W., Gabelli S.B., Goutsias J., Inoue T.* “Intracellular production of hydrogels and synthetic RNA granules by multivalent interactions”. Nature Materials (In press) bioRxiv preprint link: https://doi.org/10.1101/117572

Miao YC, Bhattacharya S, Edwards M, Cai HQ, Inoue T, Iglesias PA, Devreotes PN “Altering the threshold of an excitable signal transduction network changes cell migratory modes.” Nature Cell Biology 2017;19:329-340

Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M, Rohatgi R, Reiter JF, Ikegami K, Inoue T* “Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.” Cell 2017;168:264-279

Meier EL, Razavi S, Inoue T, Goley ED. “A novel membrane anchor for FtsZ is linked to cell wall hydrolysis in Caulobacter crescentus.” Mol Microbiol. 2016;101(2):265-80

Garcia-Gonzalo FR, Phua SC, Roberson EC, Galo Garcia III, Abedin M, Schurmans S, Inoue T*, Reiter JF.* “Ciliary Phosphoinositides Modulate Hedgehog Signaling.” Developmental Cell. 2015;34:400-409.

Miyamoto T., Rho E, Sample V., Akano H., Magari M., Ueno T., Chen M., Tokumitsu H., Zhang J., and Inoue T. “Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators” Cell Reports 11, 657-670 (2015) 

Lin B., Yin T., Wu Y.I., Inoue T. and Levchenko A. “Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration” Nature Communications 6, 6619 (2015) Note: Press release: “A digital field guide to cancer cells” 

Adams P.J., Johny M.B., Dick I.E., Inoue T., and Yue D.T., “Apocalmodulin itself promotes ion channel opening and Ca2+ regulation” Cell 159 (3), 608-622 (2014) Razavi S., Su S. and Inoue T. “Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices” ACS Synthetic Biology 3 (9), 676-685 (2014) Note: “Introducing Authors” ACS Synthetic Biology

Onuma H., Komatsu T., Arita M., Hanaoka K., Ueno T., Terai T., Nagano T., and Inoue T. “Rapidly rendering cells phagocytic through a cell-surface display technique and concurrent Rac activation” Science Signaling, 7, rs4, 1-7 (2014) Note: “Cover Story” Science Signaling, “Turning cells into garbage collector” Editor’s Choice in Science, Interviewed and broadcasted by FOX45 NEWS (https://drive.google.com/file/d/0B-wUSlcBaaEsSmpmQmhIblMzTXc/view?usp=sh…), Interview for “Thought Leaders” series by Medical-News, Press release: MOLECULAR “EAT NOW” SIGNAL MAKES CELLS DEVOUR DYING NEIGHBORS, “Training Cells to Devour Dying Neighbors” highlighted in NIGMS Biomedical Beat Blog, Highlighted in 5th Annual Henrietta Lacks Memorial Lecture, “Designer Immune Cells” highlighted in 2014 Research Highlights from Johns Hopkins Medicine (https://www.youtube.com/watch?v=Lb58GUNP5Qk)

Suarez A, Ueno T, Huebner R, McCaffery JM, and Inoue T “Bin/Amphiphysin/Rvs (BAR) family members bend membranes in cells” Scientific Reports 4, (4693) 1-6 (2014)

Kobayashi T, Kim S, Lin YC, Inoue T, and Dynlacht BD. “CP110-interacting proteins, Talpid3 and Cep290, play overlapping and distinct roles in cilia assembly” Journal of Cell Biology 204, 215-229 (2014)

Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda . and Inoue T. “Genetically encoded calcium indicator illuminates calcium dynamics in cilia” Nature Methods 10, 1105-1107 (2013)Note: “Author File” Nature Methods 

Thevathasa, JV, Tan E, Hui Z, Lin YC, Li Y. Inoue T and Fivaz M. “Local positive feedback from PI3K to Ras drives cell polarization and migration” Molecular Biology of the Cell (doi:10.1091/mbc.E12-12-0905) Lin YC, Su S. and Inoue T. “Visualizing Molecular Diffusion through Passive Permeability Barriers in Cells: Conventional and Novel Approaches“ Current Opinion in Chemical Biology 2013 (doi:10.1016/j.cbpa.2013.04.027)

Lin YC, Liu TY, Razavi S and Inoue T. “Rapidly Reversible Manipulation of Molecular Activities Using Dual Chemical Dimerizers” Angewandte Chemie 52, 6450 (2013)

Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi T, and Inoue T. “Chemically-inducible diffusion trap reveals molecular sieve-like barrier at primary cilia“ Nature Chemical Biology 9, 437 (2013). Note: “Research on cilia heats up: Implications for hearing vision loss and kidney disease” Press release, Featured in “ChemBioVault”, “New cilia research could have implications for kidney disease” (American Society of Nephrology, In The Loop), “New clues about molecular composition of cilia” MIGMS Latest News, “Cilia Admit Large Cytosolic Proteins”, Kidney News, “Signaling: Shifting at Ciliary Base” News & Views, Nature Chemical Biology

DeRose R,  Miyamoto T and Inoue T. “Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology“ Pfьlgers Archiv 465, 409-417 (2013)

Miyamoto T, Razavi S, DeRose R, and Inoue T. “Synthesizing Biomolecule-based Boolean Logic Gates” ACS Synthetic Biology 2, (2), 72-82 (2013) Note: “Podcast” (ACS Synthetic Biology)